JOURNAL OF BIOLOGY AND GENETIC RESEARCH (JBGR )
E-ISSN 2545-5710
P-ISSN 2695-222X
VOL. 9 NO. 2 2023
DOI: https://doi.org/10.56201/jbgr.v9.no2.2023.pg128.141
Antimicrobial Susceptibility and Determination of ESBL Producing
Multidrug Resistance Uropathogenic E. coli and Klebsiella sp.
Isolated in some Selected Hospitals in Kano State.
Ishaq, S.A , Adeshina, G.O , Onaolapo, G.A , Yusha’u, M and Ali, M
Abstract
Background: The persistent exposure of bacterial strains to a multitude of ?-lactams has induced
a dynamic and continuous production and mutation of ?-lactamases in bacteria, expanding their
activity even against the third and fourth generation cephalosporins such as ceftazidime,
cefotaxime and cefepime and against aztreonam. The presence of ESBL has been associated with
increased mortality, longer duration of hospitalization and increased hospital cost (Hang et al.,
2019).
Aim: The study was aimed to determine ESBL producing isolates among multidrug resistance
uropathogenic isolates of E. coli and Klebsiella sp isolated from the three major hospitals in Kano
State, Nigeria. A total of one hundred and thirty-one (131) suspected Klebsiella sp. and E coli
isolates were collected from the Microbiology laboratory of all the 3 study sites i.e., Murtala
Muhammad Specialist Hospital (MMSH), Muhammad Abdullahi Wase Teaching Hospital
(MAWTH) and Aminu Kano teaching hospital (AKTH) all in Kano State, North West Nigeria.
Isolates were subjected to Gram staining, morphological and biochemical characterization as well
as MicrogenTM Gram negative Identification A system. Antibiotic susceptibility testing was
conducted using modified Kirby-Bauer disc- diffusion method.
Results: Result showed that all the isolates were susceptible to gentamicin (100%), 75% and 67%
susceptible to ciprofloxacin and amoxicillin-clavulanic acid respectively. The resistance pattern
of the isolates was observed to be cephalothin (77.5%), cefpodoxime (72.5%), ampicillin (57.5 %),
tetracycline (52.5%), sulfamethoxazole-trimethoprim (50%), ceftriaxone (40%), amoxicillin-
clavulanic acid (32.5%) and ciprofloxacin (25%). Klebsiella sp. showed higher resistance to
cephalexin (90%), ampicillin (90%), cefpodoxime (80%), and then tetracycline (60%) while
resistance of E. coli to cephalexin, cefpodoxime, and tetracycline were 73.3%, 70%, and 50%
respectively. Out of the 22 MDR isolates, 14 (6
keywords: Antibiotics, Escherichia coli, Klebsiella, Kano, susceptibility pattern
References:
Adrizain, R., Suryaningrat, F., Alam, A., Setiabudi, D. (2018). Incidence of multidrug-resistant,
extensively drug-resistant and pandrug-resistant bacteria in children hospitalized at Dr. Hasan
Sadikin general Bandung Indonesia. IOP Conference Series: Earth Environ Sci. 125: 012077.
Agbagwa, O.E., Okorafor, O.N. and Horsfall. S.J. (2022). Multidrug Resistant Pattern and Plasmid
Detection of Escherichia coli from Various Sources within the University of Port Harcourt.
Open Journal of Medical Microbiology 8; 224.
Agyepong, N., Govinden, U., Owusu-Ofori, A. and Essack, S.Y. (2018). Multidrug-resistant gram-
negative bacterial infections in a teaching hospital in Ghana. Antimicrobial Resistance
Infection Control. 7(1).
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