IIARD International Institute of Academic Research and Development

JOURNAL OF BIOLOGY AND GENETIC RESEARCH (JBGR )

E-ISSN 2545-5710
P-ISSN 2695-222X
VOL. 9 NO. 2 2023
DOI: https://doi.org/10.56201/jbgr.v9.no2.2023.pg77.96

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Phylogenetic Analysis and Genetic Diversity of Bacteria Strains isolated from oral cavity of Malnourished Children: Insights from Comparative Genomics

Abiodun Jacob Osatogbe, Prof. Ahmed Ali Farouq, Associate Prof. Daniel. D Attah, Prof. Sule Sahabi Manga

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Abstract

Molecular Characterization of Bacteria Isolated from the Oral Cavities of In-Patient Malnourished Children At Specialist Hospital, Sokoto, Nigeria. Research conducted at Sokoto State Specialist Hospital looked into oral bacteria in malnourished children. Malnutrition stems from nutrient inefficiency, and diet influences health significantly. A microbiological method of isolation involves aseptic oral swabs for examination. Samples are collected by spinning the swab across the mouth for 15–20 seconds. Self-sealing polythene bags transport specimen containers to the lab, ensuring accurate results. Isolation uses nutrient agar, sorbitol, and broth agars. Gram staining, biochemical testing detect nutritious microorganisms on the agar-spread plate. The slide is dried and examined under a microscope with an oil immersion objective lens. With molecular characterization of bacteria isolates from the oral cavity of the patients using PCR.

keywords:

Sequence Children, Oral cavity, Bacteria, Malnutrition, Isolate, Specialist Hospital and In-patient.

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References:

Ahn, J., Chen, C.Y., Hayes, R.B., 2012. Oral microbiome and oral and gastrointestinal cancer risk.
Cancer Causes Control. 23 (3), 399–404.

Ahmed, T., Haque, R., Shamsir Ahmed, A. M., Petri, W. A. Jr., and Cravioto, A. (2009). Use of
metagenomics to understand the genetic basis of malnutrition. Nutr. Rev. 67, 201–206.

Alghamdi, S. (2022). Isolation and identification of the oral bacteria and their characterization for
bacteriocin production in the oral cavity. In Saudi Journal of Biological Sciences (Vol.
29, Issue 1, pp. 318–323). https://doi.org/10.1016/j.sjbs.2021.08.096
Azusa Haruta, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Mineka Yoshikawa, Toshiki Kajihara,
Koji Yahara, Norikazu Kitamura (2022) Disinfectant Susceptibility of Third-
Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated
from the Oral Cavity of Residents of Long-Term-Care Facilities, Bacteriology
Research Article, 14 December 2022 https://doi.org/10.1128/aem.01712-22
Beisel WR, (1996). Nutrition and immune function: overview. J Nutr 126: 2611S–2615S

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