IIARD International Institute of Academic Research and Development

JOURNAL OF BIOLOGY AND GENETIC RESEARCH (JBGR )

E-ISSN 2545-5710
P-ISSN 2695-222X
VOL. 9 NO. 2 2023
DOI: https://doi.org/10.56201/jbgr.v9.no2.2023.pg108.119

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In vitro Antibacterial Activity of Psidium guajava L. (Guava) Leaf Extracts against Clinical Isolates of Some Multi drug Resistant Enteric Bacteria causing Diarrhea in Children

Ali M , Ahmed I , Yusha’u M. and Shehu A.A

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Abstract

The uses of herbal treatment are one of the possible ways to treat diseases caused by multi drug resistant bacteria. The study was aimed to screen for phytochemical and to determine antibacterial activity of aqueous and ethanol extract of Psidium guajava (L.) leaf against clinical isolates of some multi drug resistant enteric bacteria causing diarrhea in Children isolated from patients attending Murtala Muhammad Specialist Hospital Kano. Agar well diffusion method was used to determine antibacterial activity of the extract while dilution method was employed to determine the Minimum inhibitory concentration (MIC) of the extract. Preliminary phytochemical screening of the extract revealed the presence of alkaloids, flavonoids, phenol, terpenoid, anthraquinone, saponin and tannin except Steroid. The leaf has highest percentage of flavonoid (6.75%), followed by alkaloid (5.5%), phenol (3.2%), saponin (3.1), terpenoid (2.25%), tannin and anthraquinone (2.1%) each. The overall sensitivity of the isolates to the extract indicated that Klebsiella spp was the most sensitive with average zone of inhibition of 13.25 mm, followed by E. coli with 13.0 mm, Salmonella spp with 12.25 mm while least sensitivity was shown by Shigella spp with 11.38 mm. it is concluded that the leaf of Psidium guajava contained bioactive components that possess antibacterial activity.

keywords:

Antimicrobial Activities, Diarrhea, Inhibition, Phytochemical, Psidium guajava,

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References:

Abdullah, M.S, Farouk S. Nas and Muhammad Ali, (2019). Antibacterial Activity of Psidium
guajava Leaf and Stem Bark Extracts against Clinical Isolates of Staphylococcus aureus
and Salmonella typhi", International Journal of Research in Pharmacy and Biosciences,
vol. 6, no. 5, pp. 11-17, 2019.
Abdelrahim, S. I., Almagboul, A.Z., Omer, M. E. and Elegami, A. (2002). Antimicrobial activity
of Psidium guajava L. Fitoterapia 73:713-715.

Aboaba, O.O., Ezeh, A.R., Anabuike, C.L. (2011) Antimicrobial activities of some Nigerian
spices on some pathogens. Agric Biol J N Am 2(8): 1187-1193.

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